KV1.1 POT CHANNEL; RB X-200UL

Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 µL of sterile deionized water. For positive control, in Western blot using 40 ng of protein per lane. For negative control, preincubate 3-5 µg of fusion protein with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blot: 1:200 using ECL; detects ~ 86kDa band on mouse membranes; some smaller minor bands may also be present. Mature channels are glycosylasted which can lead to smeary or slightly larger bands in certain preparations. It is important to use a full range of proteinase inhbitors to obtain full length protein blots.

Immunohistochemistry on rat brain sections.

Immunoprecipitation

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Anti-Potassium Channel Kv1.1 Antibody detects level of Potassium Channel Kv1.1 & has been published & validated for use in IH, IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

GST fusion protein and a C-terminal portion of mouse Kv1.1 protein (amino acids 416-495) (Accession P16388).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes a full length Kv1.1 protein. Does not cross react with any other potassium channel antigens tested so far.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.